Fungal strain, Aspergillus clavatus MTCC1323 under solid state fermentation was found to pro
duce enzymes (protease, amylase and pectinase) having potential of degrading the biofilms of Pseudomonas
aeruginosa, Bacillus subtilis and Staphylococcus aureus. Maximum specific enzyme activities were found to be
10.0, 8.0, and 10.086 U/mg for protease, amylase and pectinase, respectively, after 7 days of incubation at
27°C. Biofilms’ degradation was analyzed through FTIR technique. Various proteins and carbohydrates were
involved in the formation of biofilms as their concentrations were reduced after enzyme mixture treatment.
The degradation of the biofilms was analyzed by viability assay using flow cytometry and fluorescence micros
copy. Maximum biofilm degradation was found against P. aeruginosa and B. subtilis biofilms and showed 82
and 75% biofilm reduction, respectively, in terms of dry cell weight. Flow cytometry viability assay results
indicated that the enzyme mixture of A. clavatus was capable of degrading the bacterial biofilms.